Infusion Cystine Agar Base, HiVeg with Hemoglobin

[所属分类:培养基配方] [发布时间:2021-4-20] [发布人:李佳颐] [阅读次数:] [返回]

Infusion Cystine Agar Base, HiVeg with Hemoglobin

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Glucose .......................................................................................10.0g
Plant infusion..............................................................................10.0g
Plant peptone No. 3.....................................................................10.0g

NaCl..............................................................................................5.0g

L -Cystine.......................................................................................1.0g
Hemoglobin solution, 2%......................................................100.0mL
pH 7.2 ± 0.2 at 25°C
Source: This medium, without hemoglobin solution, is available as a
premixed powder from HiMedia.
Preparation of Medium: Add components, except hemoglobin so-
lution, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling with frequent agitation.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 50°C. Aseptically add 100.0mL of sterile hemoglobin
solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the cultivation of Gram-negative cocci and other pathogenic
organisms.With added hemoglobin it is used for cultivation of Franci-
sella tularensis.