Hypoxanthine Agar

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hypoxanthine Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Agar............................................................................................15.0g
Peptone .........................................................................................5.0g
Hypoxanthine solution..................................................................5.0g
Beef extract...................................................................................3.0g
pH 7.0 ± 0.1 at 25°C
Hypoxanthine Solution:
Composition per 100.0mL:
Hypoxanthine................................................................................5.0g
Preparation of Hypoxanthine Solution: Add hypoxanthine to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Preparation of Medium: Add components, except hypoxanthine
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Aseptically add 100.0mL of sterile hypoxanthine solu-

tion. Mix thoroughly. Pour into sterile 15mm × 100mm Petri dishes in

25.0mL volumes.
Use: For the cultivation and differentiation of bacteria based on
hypoxanthine hydrolysis. Bacteria that hydrolyze hypoxanthine, such
as Streptomyces griseus, appear with a clear zone under and around the
colonies. Nocardia asteroides does not hydrolyze hypoxanthine.