Hyphomicrobium Strain X Broth

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hyphomicrobium Strain X Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

K 2 HPO 4 .......................................................................................1.55g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
Methylamine·HCl .........................................................................0.7g
NaH 2 PO 4 ·H 2 O...............................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
Trace elements solution .............................................................0.2mL

pH 7.2 ± 0.2 at 25°C

Trace Elements Solution:
Composition per liter:
Disodium EDTA.........................................................................50.0g
ZnSO 4 ·7H 2 O...............................................................................22.0g
CaCl 2 ·2H 2 O ................................................................................5.54g
MnCl 2 ·4H 2 O ...............................................................................5.06g
FeSO 4 ·7H 2 O..................................................................................5.0g
CoCl 2 ·6H 2 O................................................................................1.61g
CuSO 4 ·5H 2 O...............................................................................1.57g
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O....................................................................1.1g
Preparation of Trace Elements Solution : Add components to
distilled/deionized water and bring volume to 1.0L. Adjust pH to 7.0
with KOH.
Preparation of Medium:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation and maintenance of Hyphomicrobium species.