Hyphomicrobium methylovorum Medium

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hyphomicrobium methylovorum Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar............................................................................................15.0g
(NH 4 ) 2 HPO 4 ..................................................................................3.0g
NaCl..............................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
FeSO 4 ·7H 2 O.............................................................................10.0mg
MnSO 4 ·2H 2 O.............................................................................5.0mg
Tap water ......................................................................................1.0L
Methanol..................................................................................10.0mL
Vitamin mixture.........................................................................5.0mL
Vitamin Mixture:
Composition per liter:
Inositol...................................................................................200.0mg
Choline...................................................................................100.0mg
Calcium  DL -pantothenate.........................................................40.0mg
Niacin.......................................................................................40.0mg
Pyridoxine·HCl........................................................................40.0mg
Riboflavin ................................................................................40.0mg
p-Aminobenzoic acid...............................................................20.0mg
Thiamine·HCl ..........................................................................20.0mg
Biotin .........................................................................................0.2mg
Folic acid....................................................................................0.2mg
Cyanocobalamin .........................................................................2.0μg
Preparation of Vitamin Mixture:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Methanol:  Filter sterilize 10.0mL of methanol.
Preparation of Medium: Add components, except methanol and vi-
tamin mixture, to distilled/deionized water and bring volume to 985.0mL.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Asepti-
cally add 10.0mL of sterile methanol and 5.0mL of sterile vitamin mixture.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Hyphomicrobium methylovorum.