Hyperthermus butylicus Medium

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hyperthermus butylicus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1010.0mL:

NaCl............................................................................................17.0g
Pancreatic digest of casein............................................................6.0g
Sulfur, powdered...........................................................................6.0g
MgSO 4 ·7H 2 O................................................................................3.5g
MgCl 2 ·6H 2 O ...............................................................................2.75g
NiCl 2 ·6H 2 O...................................................................................2.0g
Yeast extract..................................................................................2.0g
CaCl 2 ·2H 2 O ................................................................................0.75g
KH 2 PO 4 .........................................................................................0.5g
NH 4 Cl ...........................................................................................0.5g
KCl............................................................................................0.325g
NaBr............................................................................................0.05g
H 3 BO 3 .......................................................................................0.015g
(NH 4 ) 2 SO 4 ...............................................................................10.0mg
SrCl 2 ·6H 2 O ................................................................................7.5mg
Citric acid...................................................................................5.0mg
KI...............................................................................................2.5mg
Resazurin...................................................................................1.0mg
Trace elements solution...........................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
pH 6.0–6.5 at 25°C
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7 H 2 O...............................................................................3.0g
Nitrilotriacetic acid......................................................................1.5 g
CaCl 2 ·2 H 2 O.................................................................................1.0g
NaCl..............................................................................................1.0g
MnSO 4 ·2 H 2 O...............................................................................0.5g
CoSO 4 ·7 H 2 O..............................................................................0.18g
ZnSO 4 ·7 H 2 O..............................................................................0.18g
FeSO 4 ·7 H 2 O................................................................................0.1g
NiCl 2 ·6 H 2 O..............................................................................0.025g
KAI(SO 4 ) 2 ·12 H 2 O.....................................................................0.02g
CuSO 4 ·5 H 2 O..............................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2 H 2 O.........................................................................0.01g
Na 2 SeO 3 ·5 H 2 O..........................................................................0.3mg
Preparation of Trace Elements Solution : Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Adjust pH to 6.5 with
KOH. Add remaining components. Adjust pH to 7.0 with KOH. Add
distilled/deionized water to 1.0L.
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O....................................................................................0.5g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Gas under 80% N 2 + 20% CO 2 . Autoclave for 15 min at 15 psi pres-
sure–121°C.
Preparation of Medium: Add components, except Na 2 S·9H 2 O solu-
tion, to distilled/deionized water and bring volume to 1.0L. Mix thorough-
ly. Adjust pH to 6.0–6.5 with 6N H 2 SO 4 . Sparge wih 100% N 2 . Sterilize
by bringing to 90°C for 60 min on 3 consecutive days. Immediately prior
to inoculation, add 10.0mL of sterile Na 2 S·9H 2 O solution. Mix thor-
oughly.
Use:  For the cultivation and maintenance of Hyperthermus butylicus.