Hydroxybenzoic Acid Medium

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hydroxybenzoic Acid Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
K 2 HPO 4 ·3H 2 O.............................................................................4.25g
NH 4 Cl ...........................................................................................2.0g
4-Hydroxybenzoic acid.................................................................1.0g
NaH 2 PO 4 ·H 2 O...............................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Nitrilotriacetic acid.......................................................................0.1g
FeSO 4 ·7H 2 O..............................................................................0.012g
MnSO 4 ·H 2 O...............................................................................3.0mg
ZnSO 4 ·7H 2 O..............................................................................3.0mg
CoSO 4 ........................................................................................1.0mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium:  Add 4-hydroxybenzoic acid and nitrilo-
triacetic acid to approximately 600.0mL of distilled/deionized water.
Adjust pH to 8.0 with concentrated NaOH. Add remaining compo-
nents. Mix thoroughly. Readjust pH to 7.2. Bring volume to 1.0L with
distilled/deionized water. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Bacillus species.