Hydrogenothermus hirschii Medium (DSMZ Medium 783)

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hydrogenothermus hirschii Medium
(DSMZ Medium 783)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 ·7H 2 O................................................................................7.0g
MgCl 2 ·6H 2 O .................................................................................5.5g
NaHCO 3 ........................................................................................2.0g
KCl............................................................................................. 0.65g
CaCl 2 ·2H 2 O ..................................................................................0.5g
Sulfur, powdered...........................................................................0.5g
NH 4 Cl .........................................................................................0.15g
K 2 HPO 4 .......................................................................................0.15g
NaBr..............................................................................................0.1g
Trace elements solution ...........................................................10.0mL
CaCO 3 solution..........................................................................5.0mL
pH 7.0 ± 0.2 at 25°C
CaCO 3 Solution:
Composition per 10.0mL:
CaCO 3 ...........................................................................................1.0g
Preparation of CaCO 3 Solution: Add CaCO 3 to 10.0mL of dis-
tilled/deionized water. Mix thoroughly. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to room temperature.
Trace Elements Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·2H 2 O................................................................................0.5g
CoSO 4 ·7H 2 O...............................................................................0.18g
ZnSO 4 ·7H 2 O...............................................................................0.18g
CaCl 2 ·2H 2 O...................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
NiCl 2 ·6H 2 O...............................................................................0.025g
KAl(SO 4 ) 2 ·12H 2 O.......................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·4H 2 O ..........................................................................0.01g
CuSO 4 ·5H 2 O...............................................................................0.01g
Na 2 SeO 3 ·5H 2 O...........................................................................0.3mg
Preparation of Trace Elements Solution:  Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add remaining components. Add distilled/deionized
water to 1.0L. Mix thoroughly.
Preparation of Medium: Prepare the medium aerobically Add sul-
fur to 900.0mL distilled/deionized water. Dissolve sulfur using Ultra-
Turrax dispersing instrument. Add remaining components. Bring vol-
ume to 1.0L with distilled/deionized water. Adjust pH to 7.0 using
H 2 SO 4 . Fill 20.0mL medium into 100mL serum bottles. Seal with a
rubber stopper. Change atmosphere to 80% H 2 + 20% CO 2  with an
overpressure of two atmospheres. Autoclave for 20 min at 15 psi pres-
sure–121°C. Cool to room temperature. Inject 20.0mL filter sterilized
air and 0.1mL sterile CaCO 3 solution. Shake to mix.
Use: For the cultivation of Hydrogenophilus hirschii (Hydrogenother-
mophilus hirschii).