Hydrogen-oxidizing Medium (DSMZ Medium 1003)

[所属分类:培养基配方] [发布时间:2021-4-19] [发布人:李佳颐] [阅读次数:] [返回]

Hydrogen-oxidizing Medium
(DSMZ Medium 1003)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

MgSO 4 · 7H 2 O................................................................................7.0g
NaS 2 O 3 ..........................................................................................2.0g
MES............................................................................................1.95g
MgCl 2 ·6H 2 O................................................................................0.78g
KCl..............................................................................................0.48g
CaCl 2 ·2H 2 O...................................................................................0.4g
Trace elements solution ...........................................................10.0mL
Solution A..................................................................................2.0mL
Solution B..................................................................................1.5mL
pH 6.0 ± 0.2 at 25°C
Solution A :
Composition per liter:
NH 4 Cl ......................................................................................100.0g
MgCl 2 ·6H 2 O..............................................................................100.0g
CaCl 2 ·2H 2 O.................................................................................40.0g
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 4.0 with
HCl.
Solution B :
Composition per liter:
K 2 HPO 4 ·3H 2 O...........................................................................200.0g
Preparation of Solution B:  Add K 2 HPO 4 ·3H 2 O to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
Na-EDTA......................................................................................0.5g
CoCl 2 ..........................................................................................0.15g
MnCl 2 ·4H 2 O .................................................................................0.1g
FeSO 4 ·7H 2 O..................................................................................0.1g
ZnCl 2 .............................................................................................0.1g
AlCl 3 ·6H 2 O.................................................................................0.04g
NaWoO 4 ·2H 2 O............................................................................0.03g
CuCl 2 ·2H 2 O................................................................................0.02g
NiSO 4 ·6H 2 O................................................................................0.02g
H 3 BO 3 .........................................................................................0.01g
Na 2 SeO 4 ......................................................................................0.01g
NaMoO 4 ·2H 2 O............................................................................0.01g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust the pH to 3.0.
Preparation of Medium: Sparge 1.0L of distilled/deionized water
with 100% CO 2 to produce anaerobic water. Add components to 1.0L
of the anaerobic water. Mix thoroughly. The pH should be 6.0. Sparge
with 100% CO 2  for 20 min. Dispense 5.0mL aliquots into sealable cul-
ture tubes. Place stopper on culture tube and crimp tube cap onto stop-
per. Autoclave for 20 min at 15 psi pressure–121°C. Add 1.0mL of O 2
to each tube before inoculation. After inoculation pressurize the tubes
with H 2 (138 KP).
Use: For the cultivation of Sulfurihydrogenibium azorense.