Acetobacterium Medium (ATCC Medium 1612)

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetobacterium Medium
(ATCC Medium 1612)
Composition per liter:
Fructose.......................................................................................10.0g
NaHCO 3 ......................................................................................10.0g
Yeast extract..................................................................................2.0g
NH 4 Cl ...........................................................................................1.0g
L- Cysteine·HCl·H 2 O .....................................................................0.5g
Na 2 S·9H 2 O....................................................................................0.5g
K 2 HPO 4 .......................................................................................0.45g
KH 2 PO 4 .......................................................................................0.33g
MgSO 4 ·7H 2 O................................................................................0.1g
Resazurin...................................................................................1.0mg
Wolfe’s mineral solution..........................................................20.0mL
Wolfe’s vitamin solution..........................................................20.0mL
pH 7.4 ± 0.2 at 25°C
Wolfe’s Mineral Solution:
Composition per liter:
MgSO 4 ·7H 2 O................................................................................3.0g
Nitrilotriacetic acid.......................................................................1.5g
NaCl..............................................................................................1.0g
MnSO 4 ·H 2 O..................................................................................0.5g
FeSO 4 ·7H 2 O..................................................................................0.1g
CoCl 2 ·6H 2 O..................................................................................0.1g
CaCl 2 .............................................................................................0.1g
ZnSO 4 ·7H 2 O.................................................................................0.1g
CuSO 4 ·5H 2 O...............................................................................0.01g
AlK(SO 4 ) 2 ·12H 2 O.......................................................................0.01g
H 3 BO 3 .........................................................................................0.01g
Na 2 MoO 4 ·2H 2 O..........................................................................0.01g
Preparation of Wolfe’s Mineral Solution: Add nitrilotriacetic
acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH
to 6.5 with KOH. Add distilled/deionized water to 1.0L. Add remain-
ing components.
Wolfe’s Vitamin Solution:
Composition per liter:
Pyridoxine·HCl........................................................................10.0mg
Thiamine·HCl............................................................................5.0mg
Riboflavin..................................................................................5.0mg
Nicotinic acid.............................................................................5.0mg
Calcium pantothenate ................................................................5.0mg
p-Aminobenzoic acid.................................................................5.0mg
Thioctic acid ..............................................................................5.0mg
Biotin .........................................................................................2.0mg
Folic acid....................................................................................2.0mg
Cyanocobalamin .....................................................................100.0μg
Preparation of Wolfe’s Vitamin Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium:  Add all components, except fructose, and
bring volume to 1.0L with distilled/deionized water. Mix thoroughly.
Equilibrate to pH 7.4 by gassing with 80% N 2 + 20% CO 2 . Distribute
into test tubes. Autoclave for 15 min at 15 psi pressure–121°C. Add
sterile anaerobic Na 2 CO 3 (0.25mL of 5% Na 2 CO 3 per 10.0mL of me-
dium) to bring the pH to 8.2. Add sterile fructose solution to give a final
concentration of 1%. If autotrophic growth is desired omit fructose and
gas with 80% H 2 + 20% CO 2 .
Use: For the cultivation and maintenance of Acetobacterium species,
Clostridium aceticum, and other bacteria that can ferment fructose to
acetic acid.