Acetobacter peroxydans Medium

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetobacter peroxydans Medium
Composition per liter:
Agar ............................................................................................15.0g
Malt extract.................................................................................15.0g
Yeast extract..................................................................................5.0g
Ethanol (50% solution)............................................................60.0mL
Ethanol Solution:
Composition per 100.0mL:
Ethanol (50% solution)..........................................................100.0mL
Preparation of Ethanol Solution: Filter sterilize.
Preparation of Medium: Add components, except ethanol solu-
tion, to distilled/deionized water and bring volume to 940.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
25°C. Add 60.0mL of sterile ethanol solution. Mix thoroughly. Asepti-
cally distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Acetobacter peroxydans
and Acetobacter pasteurianus.