Halophilic Synthetic Medium

[所属分类:培养基配方] [发布时间:2021-3-30] [发布人: ] [阅读次数:] [返回]

Halophilic Synthetic Medium

培养基配方

Composition per liter:
Glucose .........................................................................................0.1g
KNO 3 ..........................................................................................0.05g
FePO 4 ..........................................................................................0.01g
Artificial seawater..................................................................100.0mL
Artificial Seawater:
Composition per 100.0mL:
NaCl..............................................................................................2.4g
MgCl 2 ·6H 2 O..................................................................................1.1g
Na 2 SO 4 ..........................................................................................0.4g
CaCl 2 ·6H 2 O...................................................................................0.2g
KCl..............................................................................................0.07g
NaHCO 3 ......................................................................................0.02g
KBr..............................................................................................0.01g
SrCl 2 ·6H 2 O.................................................................................4.0mg
H 3 BO 3 ........................................................................................3.0mg
Na 2 SiO 3 ·9H 2 O............................................................................0.5mg
NaF.............................................................................................0.3mg
Preparation of Artificial Seawater: Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. 

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of halophilic bacteria.