Halomonas pantelleriense Medium (DSMZ Medium 752)

[所属分类:培养基配方] [发布时间:2021-3-29] [发布人: ] [阅读次数:] [返回]

Halomonas pantelleriense Medium

(DSMZ Medium 752)

培养基配方

Composition per liter:
NaCl..........................................................................................100.0g
Yeast extract................................................................................10.0g
Na 2 -citrate.....................................................................................3.0g
KCl............................................................................................... 2.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Na 2 CO 3  solution.......................................................................50.0mL
MnCl 2 solution...........................................................................1.0mL
FeSO 4  solution...........................................................................1.0mL
pH 9.0 ± 0.2 at 25°C
Na 2 CO 3 Solution:
Composition per 50.0mL:
Na 2 CO 3 .........................................................................................2.5g
Preparation of Na 2 CO 3 Solution : Add Na 2 CO 3 to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Filter sterilize.
FeSO 4 Solution:
Composition per 100.0mL:
FeSO 4 ·7H 2 O..................................................................................5.0g
Preparation of FeSO 4 Solution : Add FeSO 4 ·7H 2 O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
MnCl 2 Solution:
Composition per 100.0mL:
MnCl 2 ·4H 2 O .............................................................................0.036g
Preparation of MnCl 2 Solution : Add MnCl 2 ·4H 2 O to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 9.0. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use:  For the cultivation of Halomonas pantelleriensis (Halomonas pantelleriense).