Halobacterium pharaonis Medium

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人: ] [阅读次数:] [返回]

Halobacterium pharaonis Medium

培养基配方

Composition per liter:
NaCl..........................................................................................250.0g
Agar............................................................................................20.0g
Casamino acids...........................................................................15.0g
Trisodium citrate·2H 2 O.................................................................3.0g

Glutamic acid................................................................................2.5g

MgSO 4 ·7H 2 O................................................................................2.5g
KCl................................................................................................2.0g
pH 8.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 6.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Readjust pH to 8.5. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Natronobacterium (Halobacterium) pharaonis.