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Acetic Acid Broth
山东拓普生物工程有限公司
Shandong Tuopu Biol-Engineering Co.,Ltd
Acetic Acid Broth
Composition per liter:
Sodium acetate, anhydrous...........................................................4.5g
Yeast extract..................................................................................1.0g
Wort solution..........................................................................500.0mL
Sucrose solution.....................................................................500.0mL
Wort Solution:
Composition per 500.0mL:
Malt extract.................................................................................55.0g
Preparation of Wort Solution: Add malt extract to distilled/de-
ionized water and bring volume to 500.0mL. Mix thoroughly.
Sucrose Solution:
Composition per 500.0mL:
Sucrose........................................................................................50.0g
Preparation of Sucrose Solution: Add sucrose to distilled/deion-
ized water and bring volume to 500.0mL. Mix thoroughly.
Preparation of Medium: Combine components. Mix thoroughly.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation of Tetragenococcus halophilus.