Halobacterium halobium Defined Medium

[所属分类:培养基配方] [发布时间:2021-3-25] [发布人: ] [阅读次数:] [返回]

Halobacterium halobium Defined Medium

培养基配方

Composition per liter:
NaCl..........................................................................................250.0g
MgSO 4 ·7H 2 O..............................................................................20.0g
L -Glutamic acid ............................................................................1.3g
KCl................................................................................................1.0g
Glycerol ........................................................................................1.0g
KCl................................................................................................1.0g
L -Valine.........................................................................................1.0g
L -Lysine ......................................................................................0.85g
L -Leucine......................................................................................0.8g
CaCl 2 ·7H 2 O ................................................................................0.71g
L -Serine.........................................................................................0.6g
Sodium citrate·2H 2 O.....................................................................0.5g
L -Proline .......................................................................................0.5g
L -Threonine...................................................................................0.5g
L -Alanine ....................................................................................0.43g
L -Arginine.....................................................................................0.4g
L -Methionine...............................................................................0.37g
L -Phenylalanine ..........................................................................0.26g
L -Tyrosine.....................................................................................0.2g
KH 2 PO 4 .......................................................................................0.15g
K 2 HPO 4 .......................................................................................0.15g
KNO 3 ............................................................................................0.1g
KNO 3 ............................................................................................0.1g
Glycine........................................................................................0.06g
L -Cysteine·HCl·H 2 O...................................................................0.05g
L -Isoleucine...............................................................................0.044g
FeCl 2 ·5H 2 O................................................................................2.3mg
ZnSO 4 ·7H 2 O............................................................................0.44mg
CuSO 4 ·5H 2 O..........................................................................0.005mg
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Halobacterium halobium.