Acetate Agar

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetate Agar
Composition per liter:
Meat extract ................................................................................50.0g
Glucose .......................................................................................10.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................5.0g
Sodium acetate buffer............................................................100.0mL
Tween™ 80................................................................................0.5mL
pH 5.4 ± 0.2 at 25°C
Sodium Acetate Buffer:
Composition per liter:
Sodium acetate·3H 2 O................................................................272.2g
Preparation of Sodium Acetate Buffer: Add sodium acetate to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Adjust pH to 5.4 with glacial acetic acid. Filter sterilize.
Preparation of Medium: Add components, except sodium acetate
buffer, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 5.4. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptic-
ally add 100.0mL of sterile sodium acetate buffer. Mix thoroughly.
Aseptically distribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Leuconostoc species and
Pediococcus species.