Acetamide Agar

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acetamide Agar
Composition per liter:
Agar ............................................................................................15.0g
Acetamide...................................................................................10.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .......................................................................................1.39g
KH 2 PO 4 .......................................................................................0.73g
MgSO 4 ·7H 2 O................................................................................0.5g
Phenol Red................................................................................0.012g
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH. Distribute into tubes or flasks. Autoclave for 15
min at 15 psi pressure–121°C. Cool tubes in a slanted position to pro-
duce a long slant.
Use: For the differentiation of nonfermentative Gram-negative bacte-
ria, especially Pseudomonas aeruginosa. Can be used as a confirma-
tory test for water analysis. Bacteria that deamidate acetamide turn the
medium red.