ACC Medium

[所属分类:培养基配方] [发布时间:2019-9-18] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ACC Medium
Composition per liter:
Proteose peptone.........................................................................20.0g
Agar ............................................................................................12.0g
Glycerol ........................................................................................1.5g
K 2 SO 4 ............................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................1.5g
Antibiotic solution ...................................................................10.0mL
pH 7.2 ± 0.2 at 25°C.
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide..........................................................................0.075g
Ampicillin...................................................................................0.05g
Chloramphenicol.....................................................................0.0125g
Preparation of Antibiotic Solution: Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol for-
mation and inhalation.
Preparation of Medium: Add components, except antibiotic solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic solu-
tion. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile
tubes.
Use: For the selective isolation and cultivation of fluorescent Pseudo-
monas species.