Acanthamoeba Medium

[所属分类:培养基配方] [发布时间:2019-9-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Acanthamoeba Medium
Composition per liter:
Proteose peptone.........................................................................15.0g
Glucose .......................................................................................15.0g
KH 2 PO 4 .........................................................................................0.3g
L -Methionine............................................................................14.9mg
Thiamine....................................................................................1.0mg
Biotin .........................................................................................0.2mg
Vitamin B 12 .................................................................................1.0μg
Salt solution ...............................................................................1.0mL
pH 5.5 ± 0.2 at 25°C
Salt Solution:
Composition per 100.0mL:
MgSO 4 ·7H 2 O............................................................................. 2.46g
CaCl 2 ·2H 2 O.................................................................................0.15g
FeCl 3 ...........................................................................................0.02g
Preparation of Salt Solution: Add components to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.5. Fil-
ter through Whatman paper to remove particles. Distribute into screw-
capped tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Acanthamoeba species.