Fraser Secondary Enrichment Broth

[所属分类:培养基配方] [发布时间:2021-3-4] [发布人: ] [阅读次数:] [返回]

Fraser Secondary Enrichment Broth

培养基配方

Composition per liter:
NaCl............................................................................................20.0g
Na 2 HPO 4 .....................................................................................12.0g
Beef extract...................................................................................5.0g
Proteose peptone...........................................................................5.0g
Pancreatic digest of casein............................................................5.0g
Yeast extract..................................................................................5.0g
LiCl...............................................................................................3.0g
KH 2 PO 4 .......................................................................................1.35g
Esculin..........................................................................................1.0g
Acriflavin solution...................................................................10.0mL
Ferric ammonium citrate solution............................................10.0mL
Nalidixic acid solution...............................................................1.0mL
Ferric Ammonium Citrate Solution:
Composition per 10.0mL:
Ferric ammonium citrate...............................................................0.5g
Preparation of Ferric Ammonium Citrate Solution: Add ferric ammonium citrate to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Acriflavin Solution:
Composition per 10.0mL:
Acriflavin..................................................................................0.025g
Preparation of Acriflavin Solution: Add acriflavin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Nalidixic Acid Solution:
Composition per 10.0mL:
Nalidixic acid..............................................................................0.04g
NaOH (0.1N solution)..............................................................10.0mL
Preparation of Nalidixic Acid Solution: Add nalidixic acid to 10.0mL of NaOH solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except acriflavin solution and ferric ammonium citrate solution, to distilled/deionized water and bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes in 10.0mL volumes. Autoclave for 12 min at 15 psi pressure–121°C. Cool rapidly to 25°C. Immediately prior to inoculation, aseptically add 0.1mL of sterile acriflavin solution and 0.1mL of ferric ammonium citrate solution to each tube. Mix thoroughly.
Use: For the isolation, cultivation, and enrichment of Listeria monocytogenes from foods and environmental specimens based on esculin hydrolysis. Bacteria that hydrolyze esculin appear as black colonies.