FPA Medium

[所属分类:培养基配方] [发布时间:2021-3-3] [发布人: ] [阅读次数:] [返回]

FPA Medium 

培养基配方 

Composition per liter:
Proteose peptone No. 3...............................................................20.0g
Agar............................................................................................15.0g
Pectin, citrus .................................................................................5.0g
K 2 HPO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................1.5g
Antibiotic solution ...................................................................10.0mL
pH 7.0 ± 0.2 at 25°C
Antibiotic Solution:
Composition per 10.0mL:
Cycloheximide..........................................................................0.075g
Novobiocin................................................................................0.045g
Penicillin G............................................................................75,000U
Preparation of Antibiotic Solution: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.

Caution:  Cycloheximide is toxic. Avoid skin contact or aerosol formation and inhalation.

Preparation of Medium: Add components, except agar and antibiotic solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Adjust pH to 7.0. Add agar. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile antibiotic solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and cultivation of Pseudomonas species that cause soft rot.