Fluid Thioglycollate Agar

[所属分类:培养基配方] [发布时间:2021-3-2] [发布人: ] [阅读次数:] [返回]

Fluid Thioglycollate Agar

培养基配方

Composition per liter:
Pancreatic digest of casein..........................................................15.0g
Glucose.........................................................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................2.5g
Agar............................................................................................0.75g
L- Cystine.......................................................................................0.5g
Sodium thioglycolate....................................................................0.5g
Resazurin...................................................................................1.0mg
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of anaerobic bacteria. For the cultivation of Campylobacter fetus, Campylobacter jejuni, Leptotrichia buccalis, and Streptococcus species.