Flexistipes Medium

[所属分类:培养基配方] [发布时间:2021-3-1] [发布人: ] [阅读次数:] [返回]

Flexistipes Medium

培养基配方

Composition per liter:
NaCl..........................................................................................58.35g
MgCl 2 ·6H 2 O .............................................................................37.80g
Na 2 SO 4 ........................................................................................9.72g
CaCl 2 ·2H 2 O ................................................................................7.14g
KCl..............................................................................................1.65g
Yeast extract..................................................................................1.0g
H 3 BO 3 .........................................................................................0.66g
Na 2 S·9H 2 O....................................................................................0.5g
KBr .............................................................................................0.24g
SrCl 2 ·6H 2 O .................................................................................0.17g
Na 2 HPO 4 ·2H 2 O...........................................................................0.03g
Sodium meta silicate.................................................................0.012g
NaF ...........................................................................................0.007g
KNO 3 ........................................................................................0.005g
Resazurin ...................................................................................0.5mg
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Prepare and dispense medium under 100% N 2 . Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.5 with H 2 SO 4 . Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenence of Flexistipes sinusarabici.