Flexibacter polymorphus Medium (LMG 108)

[所属分类:培养基配方] [发布时间:2020-7-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter polymorphus Medium
(LMG 108)

培养基配方
Composition per liter:
Agar ...........................................................................15.0g
Monosodium glutamate ............................................................5.0g
Tryptone.........................................................................1.0g
Casamino acids, vitamin-free ....................................................1.0g
Sodium glycerophosphate..........................................................0.1g
Vitamin B 12 ...................................................................1.0μg
Trace elements solution HO-LE ...................................................1.0mL
Seawater, filtered, aged.........................................................1.0L
Trace Elements Solution HO-LE:
Composition per liter:
H3BO3 ...........................................................................2.85g
MnCl2 ·4H2O......................................................................1.8g
Sodium tartrate..................................................................1.77g
FeSO4 ·7H2O.....................................................................1.36g
CoCl2 ·6H2O.....................................................................0.04g
CuCl2· 2H2O....................................................................0.027g
Na2MoO4 ·2H2O..................................................................0.025g
ZnCl2 ..........................................................................0.02g
Preparation of Trace Elements Solution HO-LE: Add components
to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Combine components. Mix thoroughly.
Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–
121°C.
Use: For the cultivation and maintenance of Flexibacter polymorphus.