Flexibacter Agar

[所属分类:培养基配方] [发布时间:2020-7-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Flexibacter Agar

培养基配方
Composition per liter:
Agar........................................................................15.0g
Sodium glutamate.............................................................5.0g
MgSO4 ·7H2O.................................................................1.0g
Yeast extract................................................................1.0g
Glucose solution.............................................................20.0mL
pH 7.2 ± 0.2 at 25°C
Glucose Solution:
Composition per 20.0mL:
Glucose.......................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 20.0mL. Mix thoroughly. Sparge with
100% N2 for 3–4 min. Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium:  Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 980.0mL. Mix
thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at
15 psi pressure–121°C. Aseptically add 20.0mL of sterile glucose so-
lution. Mix thoroughly. Pour into sterile Petri dishes or distribute into
sterile tubes.
Use:  For the cultivation of Flexibacter elegans.