AAHC Medium for YAK Clones

[所属分类:培养基配方] [发布时间:2019-9-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

AAHC Medium for YAK Clones
Composition per liter:
Glucose.......................................................................................20.0g
Acid hydrolysate of casein..........................................................10.0g
Yeast nitrogen base without amino acids......................................6.7g
Adenine hemi-sulfate...............................................................40.0mg
Preparation of Medium: Add glucose, yeast nitrogen base without
amino acids, and adenine hemi-sulfate to distilled/deionized water and
bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Add acid
hydrolysate of casein to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to room temperature. Asep-
tically combine the two sterile solutions. Mix thoroughly. Aseptically
distribute into sterile tubes or flasks.
Use: For the cultivation and maintenance of Saccharomyces cerevi-
siae.