A 3B Agar

[所属分类:培养基配方] [发布时间:2019-9-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

A 3B Agar
Composition per 101.5mL:
Agar base.................................................................................80.0mL
Supplement solution................................................................21.5mL
pH 6.0 ± 0.2 at 25°C
Agar Base:
Composition per liter:
Pancreatic digest of casein..........................................................17.0g
Ionagar No. 2................................................................................7.5g
NaCl..............................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
Glucose.........................................................................................2.5g
Source: Ionagar No. 2 is available from Oxoid Unipath.
Preparation of Agar Base:  Add components, except agar, to dis-
tilled/deionized water and bring volume to 1.0L. Adjust pH to 5.5. Add
agar. Mix thoroughly. Gently heat and bring to boiling. Distribute into
screw-capped bottles in 80.0mL volumes. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C.
Supplement Solution:
Composition per 21.5mL:
Horse serum-urea solution.......................................................20.0mL
Penicillin solution......................................................................1.0mL
L -Cysteine·HCl·H 2 O solution....................................................0.5mL
Preparation of Supplement Solution:  Aseptically combine com-
ponents. Mix thoroughly.
Horse Serum-Urea Solution:
Composition per 40.0mL:
Urea...............................................................................................0.2g
Horse serum, unheated.............................................................40.0mL
© 2010 by Taylor and Francis Group, LLC
A 7 Agar, Modified  13
Preparation of Horse Serum-Urea Solution: Add urea to
40.0mL of horse serum. Mix thoroughly. Filter sterilize.
Penicillin Solution:
Composition per 10.0mL:
Penicillin G.......................................................................1,000,000U
Preparation of Penicillin Solution: Add penicillin to distilled/de-
ionized water and bring volume to 10.0mL. Mix thoroughly. Filter ster-
ilize.
L -Cysteine·HCl·H 2 O Solution:
Composition per 10.0mL:
L- Cysteine·HCl·H 2 O......................................................................0.2g
Preparation of  L -Cysteine·HCl·H 2 O Solution: Add  L-
cysteine·HCl·H 2 O to distilled/deionized water and bring volume to
10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Aseptically combine 80.0mL of cooled,
sterile agar base and 21.5mL of sterile supplement solution. Mix thor-
oughly.
Use: For the cultivation of Ureaplasma urealyticum from urine. Also
used for the cultivation of other Ureaplasma species.