Escherichia Medium (ATCC Medium 62)

[所属分类:培养基配方] [发布时间:2020-7-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Escherichia Medium
(ATCC Medium 62)

培养基配方
Composition per 700.0mL:
Agar ......................................................................12.5g
Casein hydrolysate..........................................................6.0g
Glycerol ...................................................................2.0g
K2HPO4 .....................................................................0.2g
MgSO4 ·7H2O................................................................0.2g
L- Asparagine .............................................................0.15g
FeSO4 ·7H2O...............................................................5.0mg
Vitamin B 12 ..............................................................0.4mg
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components, except glycerol, agar,
and vitamin B 12 , to distilled/deionized water and bring volume to
500.0mL. Adjust pH to 7.2. Mix thoroughly. Gently heat and bring to
boiling. Filter through Whatman #1 filter paper. Cool to 25°C. Add agar
and glycerol. Bring volume to 1.0L with distilled/deionized water. Mix
thoroughly. Gently heat and bring to boiling. Readjust pH to 7.2. Add vi-
tamin B 12 . Mix thoroughly. Distribute into tubes in 10.0mL volumes.
Autoclave for 15 min at 15 psi pressure–121°C. Allow tubes to cool in a
slanted position.
Use: For the cultivation and maintenance of Escherichia coli.