A 1 Minimal Medium

[所属分类:培养基配方] [发布时间:2019-9-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

A 1 Minimal Medium
Composition per liter:
L- Asparagine .................................................................................5.0g
(NH 4 ) 2 SO 4 .....................................................................................5.0g
Sodium pyruvate...........................................................................5.0g
MgSO 4 ·7H 2 O................................................................................2.0g
Spermadine·3HCl......................................................................0.125g
L- Asparagine .................................................................................0.1g
L- Isoleucine...................................................................................0.1g
L- Methionine.................................................................................0.1g
L- Phenylalanine.............................................................................0.1g
L- Valine .........................................................................................0.1g
L- Leucine.....................................................................................0.05g
KH 2 PO 4 .....................................................................................0.013g
FeCl 3 ·6H 2 O................................................................................2.7mg
CaCl 2 ..........................................................................................1.1mg
Cyanocobalamin ........................................................................1.0mg
Tris(hydroxymethyl)aminomethane
buffer (0.01M solution, pH 7.6).............................................1.0L
pH 7.6 ± 0.2 at 25°C
Preparation of Medium: Add solid components to 1.0L of Tris
buffer. Mix thoroughly. Filter sterilize. Aseptically distribute into tubes
or flasks.
Use: For the cultivation of Myxococcus xanthus.