A-1 Medium (BAM M1)

[所属分类:培养基配方] [发布时间:2019-9-16] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

A-1 Medium
(BAM M1)
Composition per liter:
Pancreatic digest of casein..........................................................20.0g
Lactose..........................................................................................5.0g
NaCl..............................................................................................5.0g
Salicin...........................................................................................0.5g
Triton™ X-100..........................................................................1.0mL
pH 6.9 ± 0.1 at 25°C
Source: This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 6.9.
Distribute into test tubes containing an inverted Durham tube. Medium
may be cloudy prior to autoclaving. Autoclave for 10 min at 15 psi
pressure–121°C.
Use: For the detection of fecal coliforms in foods and waters by a
most-probable-number (MPN) method. Multiple dilutions of samples
(3, 5, or 10 replicates per dilution) are added to tubes containing A-1
medium. After incubation, test tubes with gas accumulation in the Dur-
ham tubes are scored positive and those with no gas as negative. A
MPN table is consulted to determine the most probable number of fecal
coliforms.