​Enriched Nutrient Broth

[所属分类:培养基配方] [发布时间:2020-7-7] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enriched Nutrient Broth

培养基配方
Composition per liter:
Beef heart, infusion from.................................................250.0g
Tryptose....................................................................5.0g
Pancreatic digest of gelatin...............................................3.38g
NaCl........................................................................2.5g
Yeast extract...............................................................2.5g
Beef extract...............................................................2.02g
pH 7.2 ± 0.1 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the cultivation and maintenance of Bacillus licheniformis, Bacil-
lus polymyxa, Bacillus subtilis, Escherichia coli, Listonella anguillarum,
Micrococcus luteus, Pseudomonas aeruginosa, Salmonella choleraesuis,
Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus spe-
cies, and Vibrio cholerae.