Enriched Isonema Medium

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enriched Isonema Medium

培养基配方
Composition per 1111.0mL:
Natural seawater .........................................................1.0L
Horse serum, inactivated...............................................100.0mL
Enrichment solution.....................................................10.0mL
Vitamin solution.........................................................1.0mL
Enrichment Solution:
Composition per liter:
NaNO3 ..................................................................4.667g
Na2SiO3 ·9H2O............................................................3.0g
Sodium glycerophosphate.................................................0.667g
EDTA·2H2O..............................................................0.553g
H3BO3 ..................................................................0.38g
Fe(NH4)2(SO4)2 ·6H2O .................................................0.234g
MnSO4 ·4H2O...........................................................0.054g
FeCl3 ·6H2O...........................................................0.016g
ZnSO4 ·7H2O...........................................................7.3mg
CoSO4 ·7H2O...........................................................1.6mg
Preparation of Enrichment Solution: Add Na2SiO3 ·9H2O to dis-
tilled/deionized water. Mix thoroughly. Neutralize with 1N HCl. Add
500.0mL of distilled/deionized water. Mix thoroughly. Add remaining
components and bring volume to 1.0L with distilled/deionized water. Mix
thoroughly. Filter sterilize.
Vitamin Solution:
Composition per liter:
Thiamine...............................................................0.1g
Vitamin B 12 .........................................................2.0mg
Biotin ...............................................................1.0mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Allow natural seawater to age for 2
months. Filter sterilize. Aseptically add 10.0mL of sterile enrichment
solution and 1.0mL of sterile vitamin solution. Mix thoroughly. Prior
to inoculation, aseptically add 100.0mL of heat-inactivated horse se-
rum. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Diplonema species, Gymnophrydium mari-
num, Isonema species, and Rhynchopus species.