Enriched Cytophaga Agar Medium (DSMZ Medium 1133)

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Enriched Cytophaga Agar Medium
(DSMZ Medium 1133)

培养基配方
Composition per liter:
Agar .........................................................................15.0g
Tryptone ......................................................................2.0g
Beef extract ..................................................................0.5g
Yeast extract .................................................................0.5g
Sodium acetate ................................................................0.2g
pH 7.3 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.3.
Gently heat while stirring and bring to boiling. Distribute into tubes or
flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into Petri
dishes or aseptically distribute into sterile tubes. For soft agar for the
maintenance of active cultures reduce agar content to 4.0g/lL. Dis-
pense in 3–4mL amounts in 7mL screw-capped bottles (bijou bottles).
Use: For the cultivation of Cytophaga spp.