Endo HiVeg Agar Base

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Endo HiVeg Agar Base

培养基配方
Composition per liter:
Agar .........................................................................12.0g
Plant peptone.................................................................10.0g
Lactose.......................................................................10.0g
K2HPO4 ........................................................................3.5g
Na2SO3 ........................................................................2.5g
Basic Fuchsin solution........................................................4.0mL
pH 7.5 ± 0.2 at 25°C
Source:  This medium, without Basic Fuchsin solution, is available as
a premixed powder from HiMedia.
Basic Fuchsin Solution:
Composition per 10.0mL:
Basic Fuchsin.................................................................1.0g
Ethanol (95% solution)......................................................10.0mL
Preparation of Basic Fuchsin Solution: Add Basic Fuchsin to
10.0mL of ethanol. Mix thoroughly.
Caution: Basic Fuchsin is a potential carcinogen and care must be
taken to avoid inhalation of the powdered dye and contact with the
skin.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
45°–50°C. Pour into sterile Petri dishes. Swirl flask while pouring
plates to keep precipitate in suspension. Protect from the light.
Use: For the selective isolation, cultivation, and differentiation of coli-
form and other enteric microorganisms based on their ability to ferment
lactose. Lactose-fermenting bacteria appear as dark red colonies with a
gold metallic sheen. Lactose-nonfermenting bacteria appear as colorless
or translucent colonies.