ENB Agar

[所属分类:培养基配方] [发布时间:2020-7-6] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ENB Agar

培养基配方
Composition per liter:
Agar......................................................................15.0g
Pancreatic digest of gelatin...............................................5.0g
NaCl.......................................................................5.0g
Peptone ...................................................................5.0g
K2HPO4 ....................................................................3.5g
Beef extract...............................................................3.0g
KH2PO4 ....................................................................1.5g
Glucose solution.........................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Glucose Solution:
Composition per liter:
Glucose..................................................................1.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for
15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 6.8. Auto-
clave for 15 min at 15 psi pressure–121°C. Aseptically add 10.0mL of
sterile glucose solution. Mix thoroughly. Pour into sterile Petri dishes
or distribute into sterile tubes.
Use:  For the cultivation of Escherichia coli.