EMB Agar (Eosin Methylene Blue Agar)

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EMB Agar
(Eosin Methylene Blue Agar)

培养基配方
Composition per liter:
Agar..........................................................................13.5g
Pancreatic digest of casein...................................................10.0g
Lactose.......................................................................5.0g
Sucrose.......................................................................5.0g
K2HPO4 .......................................................................2.0g
Eosin Y.......................................................................0.4g
Methylene Blue..............................................................0.065g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the isolation, cultivation, and differentiation of Gram-nega-
tive enteric bacteria based on lactose fermentation. Bacteria that fer-
ment lactose, especially the coliform bacterium Escherichia coli,
appear as colonies with a green metallic sheen or blue-black to brown
color. Bacteria that do not ferment lactose appear as colorless or trans-
parent, light purple colonies.