EE Broth, Mossel (Enterobacteriaceae Enrichment Broth, Mossel)

[所属分类:培养基配方] [发布时间:2020-7-3] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EE Broth, Mossel
(Enterobacteriaceae Enrichment Broth, Mossel)

培养基配方
Composition per liter:
Enzymatic hydrolysate of protein......................................10.0g
Na2HPO4 ...............................................................8.0g
Glucose ...............................................................5.0g
KH2PO4 ................................................................2.0g
Oxgall.................................................................0.1g
Brilliant Green ....................................................0.0135g
pH 7.2 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into flasks
in 120.0mL volumes. Gently heat at 100°C for 30 min. Do not auto-
clave. Cool rapidly to 25°C.
Use: For the cultivation and enrichment of members of the Enteroba-
teriaceae in the examination of foods and animal feed. Used in con-
junction with tryptone soy broth. Bacteria belonging to the Enterobac-
teriaceae turn this medium turbid and yellow-green.