EC HiVeg Broth

[所属分类:培养基配方] [发布时间:2020-7-2] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

EC HiVeg Broth
培养基配方
Composition per liter:
Plant hydrolysate no. 1.........................................................20.0g
Lactose..........................................................................5.0g
NaCl.............................................................................5.0g
K2HPO4 ..........................................................................4.0g
KH2PO4 .........................................................................1.5g
Synthetic detergent.............................................................1.5g
pH 6.9 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Distribute into test
tubes that contain an inverted Durham tube. Autoclave for 15 min at 15
psi pressure–121°C. Cool broth as quickly as possible.
Use: For the cultivation and differentiation of coliform bacteria at 37°C
and of Escherichia coli at 45.5°C. Recommended for selective enumera-
tion of presumptive Escherichia coli by MPN technique. For the selective
enumeration of faecal and nonfaecal coliforms in water, wastewater, and
shell fish.