Dichloran 18% Glycerol Agar (DG18 Agar) (BAM M184)

[所属分类:培养基配方] [发布时间:2020-6-17] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dichloran 18% Glycerol Agar
(DG18 Agar)
(BAM M184)

培养基配方
Composition per liter:
Glycerol .....................................................................220.0g
Agar ..........................................................................15.0g
Glucose .......................................................................10.0g
Peptone.........................................................................5.0g
KH2PO4 .........................................................................1.0g
MgSO4 ·7H2O....................................................................0.5g
Dichloran .....................................................................2.0mg
Chloramphenicol ................................................................0.1g
pH 5.6 ± 0.2 at 25°C
Preparation of Medium: Add components, except glycerol, to dis-
tilled/deionized water and bring volume to 950.0mL. Mix thoroughly.
Gently heat and bring to boiling. Cool to 50°C. Add 220.0g of glycerol.
Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. The
final pH should be 5.6 and the final a w should be 0.955. Pour into sterile
Petri dishes or distribute into sterile tubes.
Use : Used as a general purpose medium for the enumeration of molds
from foods. For the enumeration and isolation of xerophilic molds
from dried and semidried foods.This medium is preferred when the a w
of the analyzed food is 0.95 or lower. The low water activity of this
medium reduces interference by bacteria and fast-growing fungi.