Dextrose Soil Agar (DSA)

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dextrose Soil Agar
(DSA)

培养基配方
Composition per liter:
Soil.....................................................................150.0g
Agar......................................................................20.0g
Glucose....................................................................5.0g
Preparation of Medium: Add soil to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Autoclave for 60 min at 15 psi
pressure–121°C. Filter through Whatman #1 filter paper. Bring volume
of filtrate to 1.0L with distilled/deionized water. Mix thoroughly. Add
agar and glucose. Gently heat and bring to boiling. Distribute into tubes
or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into
sterile Petri dishes or leave in tubes
Use:  For the cultivation and maintenance of Chaetomium globosum.