Dextrose HiVeg Broth with Blood

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dextrose HiVeg Broth with Blood

培养基配方
Composition per liter:
Plant hydrolysate No. 1................................................10.0g
NaCl....................................................................5.0g
Glucose ................................................................5.0g
Plant extract...........................................................3.0g
Sheep blood, defibrinated.............................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium wtihout sheep blood is available as a premixed
powder from HiMedia.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 50.0mL sterile blood.
Aseptically distribute into tubes or flasks.
Use: For the cultivation and maintenance of a wide variety of microor-
ganisms.
Dextrose HiVeg Peptone Agar
Composition per liter:
Plant peptone ..........................................................20.0g
Agar....................................................................15.0g
Glucose.................................................................10.0g
NaCl.....................................................................5.0g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of a wide variety of microor-
ganisms.