Dextrose HiVeg Agar with Blood

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dextrose HiVeg Agar with Blood

培养基配方
Composition per liter:
Agar .........................................................................15.0g
Glucose ......................................................................10.0g
Plant hydrolysate No. 1.......................................................10.0g
NaCl...........................................................................5.0g
Plant extract .................................................................3.0g
Sheep blood, defibrinated....................................................50.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium wtihout sheep blood is available as a premixed
powder from HiMedia.
Preparation of Medium: Add components, except sheep blood, to
distilled/deionized water and bring volume to 950.0mL. Mix thorough-
ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add 50.0mL sterile blood.
Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of a wide variety of microorgan-
isms.