Dextrose Ascitic Fluid Semisolid Agar

[所属分类:培养基配方] [发布时间:2020-6-12] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Dextrose Ascitic Fluid Semisolid Agar

培养基配方
Composition per liter:
Pancreatic digest of casein.................................................2.66g
NaCl........................................................................1.33g
Agar ........................................................................0.5g
Phenol Red..................................................................4.8mg
Ascitic fluid..............................................................50.0mL
Glucose solution ..........................................................15.0mL
pH 7.4 ± 0.2 at 25°C
Glucose Solution:
Composition per 15.0mL:
Glucose ....................................................................3.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 15.0mL. Mix thoroughly. Filter steril-
ize.
Preparation of Medium: Add components, except ascitic fluid and
glucose solution, to distilled/deionized water and bring volume to
935.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically
add sterile ascitic fluid and glucose solution. Mix thoroughly. Asepti-
cally distribute into sterile tubes.
Use: For the isolation and cultivation of microorganisms from spinal
fluid.