Desulfuromonas Medium

[所属分类:培养基配方] [发布时间:2020-6-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfuromonas Medium

培养基配方
Composition per 1003.0mL:
Solution A.............................................................950.0mL
Solution C..............................................................40.0mL
Solution D..............................................................10.0mL
Solution B...............................................................3.0mL
pH 7.2 ± 0.2 at 25°C
Solution A:
Composition per 950.0mL:
NaCl.....................................................................20.0g
Disodium  DL -malate ....................................................2.66g
MgCl2 ·6H2O..............................................................2.0g
Na2SO4 ..................................................................1.77g
MgSO4 ·7H2O..............................................................1.0g
KH2PO4 ...................................................................0.5g
Yeast extract.............................................................0.5g
NH4Cl ....................................................................0.3g
CaCl2 ·2H2O..............................................................0.1g
Resazurin ...............................................................1.0mg
Trace elements solution SL-4 ...........................................10.0mL
Trace Elements Solution SL-4:
Composition per liter:
EDTA......................................................................0.5g
FeSO4 ·7H2O..............................................................0.2g
Trace elements solution SL-6 ..........................................100.0mL
Trace Elements Solution SL-6:
Composition per liter:
MnCl2 ·4H2O..............................................................0.5g
H3BO3 ....................................................................0.3g
CoCl2 ·6H2O .............................................................0.2g
ZnSO4 ·7H2O..............................................................0.1g
Na2MoO4 ·2H2O ..........................................................0.03g
NiCl2 ·6H2O.............................................................0.02g
CuCl2 ·2H2O ............................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gas
under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Trace Elements Solution SL-4:  Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly.
Preparation of Solution A : Add components to distilled/deionized
water and bring volume to 950.0mL. Mix thoroughly. Adjust pH to 6.0
with 2N NaOH. Gently heat and bring to boiling. Continue boiling for
3–4 min. Allow to cool to room temperature while gassing under 100%
N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution B:
Composition per 3.0mL:
Ethanol....................................................................0.3mL
Preparation of Solution B : Add ethanol to distilled/deionized wa-
ter and bring volume to 3.0mL. Sparge under 100% N2 . Autoclave for
15 min at 15 psi pressure–121°C.
Solution C:
Composition per 40.0mL:
NaHCO3 ....................................................................1.85g
Preparation of Solution C : Add NaHCO3 to distilled/deionized
water and bring volume to 40.0mL. Mix thoroughly. Gas under 80% N2
+ 20% CO2 . Filter sterilize.
Solution D:
Composition per 10.0mL:
Na2S·9H2O.................................................................0.3g
Preparation of Solution D : Add Na2S·9H2O to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Gas under 100%
N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: To 950.0mL of sterile solution A, asepti-
cally and anaerobically add 3.0mL of sterile solution B, 40.0mL of
sterile solution C, and 10.0mL of sterile solution D. Mix thoroughly.
Aseptically and anaerobically distribute into tubes or flasks.
Use: For the cultivation and maintenance of Desulfuromonas species.