Desulfovibrio alcoholovorans Medium

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfovibrio alcoholovorans Medium

培养基配方
Composition per liter:
Solution A...................................................................980.0mL
Solution B....................................................................10.0mL
Solution C....................................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Solution A:
Composition per 980.0mL:
MgSO4 ·7H2O.....................................................................2.0g
1,2-Propanediol..................................................................1.5g
Na2SO4 ..........................................................................1.0g
NH4Cl ...........................................................................1.0g
Yeast extract....................................................................1.0g
K2HPO4 ..........................................................................0.5g
CaCl2 ·2H2O.....................................................................0.1g
Na2SeO3 ·5H2O..................................................................3.0mg
Resazurin ......................................................................1.0mg
Preparation of Solution A:  Add components to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly. Gently heat and
bring to boiling. Continue boiling for 3–4 min. Allow to cool to room
temperature while gassing under 100% N2 .
Solution B:
Composition per 10.0mL:
FeSO4 ·7H2O.....................................................................0.5g
Preparation of Solution B:  Add FeSO4 ·7H2O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly. Gas under
100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Solution C:
Composition per 10.0mL:
Ascorbic acid.....................................................................0.1g
Sodium thioglycolate..............................................................0.1g
Preparation of Solution C:  Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly. Gas under 100%
N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: To 980.0mL of cooled solution A, anaer-
obically add 10.0mL of solution B and 10.0mL of solution C. Mix thor-
oughly. Adjust pH to 7.8 with NaOH. Distribute into tubes or flasks.
During distribution, swirl the medium to keep the precipitate in suspen-
sion. Autoclave for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation and maintenance of Desulfovibrio alcoholo-
vorans .