Desulfotomaculum spp. Medium I (DSMZ Medium 63a)

[所属分类:培养基配方] [发布时间:2020-6-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfotomaculum spp. Medium I
(DSMZ Medium 63a)

培养基配方
Composition per liter:
Soultion A...............................................................980.0mL
Solution B................................................................10.0mL
Solution C................................................................10.0mL
pH 6.5–7.0 at 25°C
Solution A:
Composition per 980.0mL:
Na-pyruvate ...............................................................5.0g
Na-acetate.................................................................2.0g
MgSO4 ·7H2O...............................................................2.0g
Yeast extract..............................................................1.0g
NH4Cl .....................................................................1.0g
Na2SO4 ....................................................................1.0g
K2HPO4 ....................................................................0.5g
CaCl2 ·2H2O ..............................................................0.1g
Resazurin ................................................................1.0mg
Preparation of Solution A: Add components to distilled/deionized
water and bring volume to 980.0mL. Mix thoroughly.
Solution B:
Composition per 10.0mL:
FeSO4 ·7H2O...............................................................0.5g
Preparation of Solution B: Add FeSO4 ·7H2O to distilled/deion-
ized water and bring volume to 10.0mL. Mix thoroughly.
Solution C:
Composition per 10.0mL:
Na-thioglycolate ..........................................................0.1g
Ascorbic acid..............................................................0.1g
Preparation of Solution C: Add components to distilled/deionized
water and bring volume to 10.0mL. Mix thoroughly.
Preparation of Medium: Bring solution A to a boil for a few min-
utes. Cool to room temperature while gassing with oxygen-free N2  gas.
Add solutions B and C. Adjust pH to 6.5–7.0. Immediately distribute
under N2  into anaerobic tubes. During distribution continuously swirl
the medium to keep the grey precipitate suspended. Autoclave for 15
min at 15 psi pressure–121°C.
Use: For the cultivation of Desulfotomaculum spp.