Desulfotomaculum alkaliphilum Medium (DSMZ Medium 866)

[所属分类:培养基配方] [发布时间:2020-6-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfotomaculum alkaliphilum Medium
(DSMZ Medium 866)

培养基配方
Composition per liter:
NaHCO3 .......................................................................8.0g
Na2SO4 .......................................................................5.0g
Na-formate....................................................................5.0g
NaCl..........................................................................5.0g
Yeast extract.................................................................1.5g
NH4Cl ........................................................................1.0g
Na2CO3 .......................................................................0.5g
Na2S·9H2O....................................................................0.5g
KH2PO4 .......................................................................0.2g
KCl...........................................................................0.2g
MgCl2 ·6H2O..................................................................0.1g
Vitamin solution............................................................10.0mL
Trace elements solution SL-10 ...............................................1.0mL
pH 8.7–9.0 at 25°C
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl..............................................................10.0mg
Thiamine-HCl·2H2O...........................................................5.0mg
Riboflavin ..................................................................5.0mg
Nicotinic acid...............................................................5.0mg
D -Ca-pantothenate...........................................................5.0mg
p-Aminobenzoic acid..........................................................5.0mg
Lipoic acid .................................................................5.0mg
Biotin ......................................................................2.0mg
Folic acid...................................................................2.0mg
Vitamin B 12 ................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H2 + 20% CO2 . Filter sterilize.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O..................................................................1.5g
CoCl2 ·6H2O ..............................................................190.0mg
MnCl2 ·4H2O...............................................................100.0mg
ZnCl2 .....................................................................70.0mg
Na2MoO4 ·2H2O ............................................................36.0mg
NiCl2 ·6H2O...............................................................24.0mg
H3BO3 .....................................................................6.0mg
CuCl2 ·2H2O ..............................................................2.0mg
HCl (25% solution)........................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Sparge with 80% N2 + 20% CO2 . Autoclave for 15 min at 15
psi pressure–121°C.
Preparation of Medium: Prepare and dispense medium anaerobi-
cally under N2 . Add components, except NaHCO3 and Na2S·9H2O, to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Gently heat and bring to boiling. Boil for 5 min. Cool to room temper-
ature while sparging with 100% N2 . Add NaHCO3 and Na2S·9H2O.
Mix thoroughly. Distribute into anaerobe tubes or bottles. Autoclave
for 15 min at 15 psi pressure–121°C.
Use:  For the cultivation of Desulfotomaculum alkaliphilum.