Desulfonatronum Medium (DSMZ Medium 813)

[所属分类:培养基配方] [发布时间:2020-6-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfonatronum Medium
(DSMZ Medium 813)

培养基配方
Composition per 1010.0mL:
NaCl..........................................................................10.0g
Na2SO4 ........................................................................5.0g
Na2CO3 ........................................................................3.5g
NH4Cl .........................................................................1.0g
Yeast extract..................................................................1.0g
Na2S·9H2O.....................................................................0.5g
KH2PO4 ........................................................................0.2g
KCl............................................................................0.2g
MgCl2 ·6H2O ..................................................................0.1g
Resazurin.....................................................................0.5mg
Na-formate solution..........................................................10.0mL
Vitamin solution.............................................................10.0mL
Trace elements solution SL-10.................................................1.0mL
pH 8.9 ± 0.2 at 25°C
Na-Formate Solution:
Composition per 10.0mL:
Na-formate....................................................................4.0g
Preparation of Na-Formate Solution:  Add Na-formate to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Vitamin Solution:
Composition per liter:
Pyridoxine-HCl..............................................................10.0mg
Thiamine-HCl·2H2O...........................................................5.0mg
Riboflavin ..................................................................5.0mg
Nicotinic acid...............................................................5.0mg
D -Ca-pantothenate...........................................................5.0mg
p-Aminobenzoic acid..........................................................5.0mg
Lipoic acid .................................................................5.0mg
Biotin ......................................................................2.0mg
Folic acid...................................................................2.0mg
Vitamin B 12 ................................................................0.1mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 1.0L. Mix thoroughly. Sparge
with 80% H2 + 20% CO2 . Filter sterilize.
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O.................................................................1.5g
CoCl2 ·6H2O .............................................................190.0mg
MnCl2 ·4H2O..............................................................100.0mg
ZnCl2 .....................................................................70.0mg
Na2MoO4 ·2H2O ............................................................36.0mg
NiCl2 ·6H2O...............................................................24.0mg
H3BO3 ......................................................................6.0mg
CuCl2 ·2H2O ...............................................................2.0mg
HCl (25% solution).........................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Preparation of Medium: Prepare and dispense medium under an
oxygen-free atmosphere of 100% N2 . Add components, except Na-for-
mate solution and Na2S·9H2O, to distilled/deionized water and bring
volume to 1.0L. Mix thoroughly. Sparge with 100% N2 for 30 min. Add
the Na2S·9H2O. Mix thoroughly. Adjust pH to 8.8–9.0. Dispense into
Hungate tubes under 100% N2 . Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C. Aseptically and anaerobically add Na-for-
mate solution, 0.1mL per 10.0mL medium.
Use:  For the cultivation of Desulfonatronum lacustre