Desulfoluna Medium (DSMZ Medium 1099)

[所属分类:培养基配方] [发布时间:2020-6-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfoluna Medium
(DSMZ Medium 1099)

培养基配方
Composition per liter:
NaCl.........................................................................20.0g
MgSO4 ·7H2O..................................................................2.0g
NH4Cl ........................................................................1.0g
Na2SO4 .......................................................................1.0g
KH2PO4 .......................................................................0.5g
Yeast extract.................................................................0.5g
CaCl2 ·2H2O..................................................................0.1g
Resazurin ...................................................................1.0mg
L -Cysteine·HCl solution...................................................10.0mL
Na-lactate, 60% solution.....................................................2.9mL
Trace elements solution SL-10 ...............................................1.0mL
pH 6.5 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O..................................................................1.5g
CoCl2 ·6H2O...............................................................190.0mg
MnCl2 ·4H2O ..............................................................100.0mg
ZnCl2 ......................................................................70.0mg
Na2MoO4 ·2H2O..............................................................36.0mg
NiCl2 ·6H2O................................................................24.0mg
H3BO3 .......................................................................6.0mg
CuCl2 ·2H2O.................................................................2.0mg
HCl (25% solution)..........................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Sparge with 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
L -Cysteine·HCl Solution:
Composition per 10.0mL:
L -Cysteine·HCl ..............................................................0.5g
Preparation of  L -Cysteine Solution : Add  L -cysteine·HCl to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Sparge with 100% N2 . Autoclave under 100% N2 for 15 min at 15 psi
pressure–121°C.
Preparation of Medium:  Add components, except cyseine solu-
tion, to distilled/deionized water and bring volume to 990.0mL. Gently
heat and bring to boiling. Boil for 1 min. Mix thoroughly. Cool to room
temperature while sparging with 100% N2 . Dispense into culture ves-
sels under an atmosphere of 100% N2 . Autoclave for 15 min at 15 psi
pressure–121°C. Aseptically add 10.0mL cysteine solution. Adjust the
final pH of the medium to 6.5.
Use: For the cultivation of Desulfoluna spp.