Desulfohalobium Medium

[所属分类:培养基配方] [发布时间:2020-6-8] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Desulfohalobium Medium

培养基配方
Composition per 1010.0mL:
NaCl......................................................................100.0g
MgCl2 ·6H2O ..............................................................20.0g
KCl.........................................................................4.0g
Na2SO4 .....................................................................3.0g
CaCl2 ·2H2O ...............................................................2.7g
NH4Cl ......................................................................1.0g
Sodium acetate..............................................................1.0g
Trypticase™.................................................................1.0g
Yeast extract...............................................................1.0g
K2HPO4 .....................................................................0.3g
KH2PO4 .....................................................................0.3g
Sodium ( L )-lactate .......................................................2.5g
Resazurin..................................................................1.0mg
Na2SeO3 ·5H2O............................................................3.0μg
Na2S·9H2O solution.......................................................10.0mL
Trace elements solution SL-10..............................................1.0mL
pH 7.0 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl2 ·4H2O................................................................1.5g
CoCl2 ·6H2O.............................................................190.0mg
MnCl2 ·4H2O ............................................................100.0mg
ZnCl2 ....................................................................70.0mg
Na2MoO4 ·2H2O............................................................36.0mg
NiCl2 ·6H2O..............................................................24.0mg
H3BO3 .....................................................................6.0mg
CuCl2 ·2H2O...............................................................2.0mg
HCl (25% solution)........................................................10.0mL
Preparation of Trace Elements Solution SL-10: Add FeCl2 ·4H2O
to 10.0mL of HCl solution. Mix thoroughly. Add distilled/deionized
water and bring volume to 1.0L. Add remaining components. Mix thor-
oughly. Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Na2S·9H2O Solution:
Composition per 10.0mL:
Na2S·9H2O................................................................1.0mg
Preparation of Na2S·9H2O Solution:  Add Na2S·9H2O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Add components, except Na2S·9H2O so-
lution, to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Gas under 100% N2 . Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically and anaerobically add 10.0mL of sterile
Na2S·9H2O solution. Mix thoroughly. Aseptically and anaerobically
distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Methanohalophilus ore-
gonense .